The Euplotes telomerase subunit p43 stimulates enzymatic activity and processivity in vitro
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منابع مشابه
The Euplotes telomerase subunit p43 stimulates enzymatic activity and processivity in vitro.
Telomerase is a reverse transcriptase that synthesizes telomeric DNA repeats at the ends of eukaryotic chromosomes. Although it is minimally composed of a conserved catalytic protein subunit (TERT) and an RNA component, additional accessory factors present in the holoenzyme play crucial roles in the biogenesis and function of the enzyme complex. Telomerase from the ciliate Tetrahymena can be re...
متن کاملdGTP-dependent processivity and possible template switching of euplotes telomerase.
We have measured the processivity of telomeric DNA extension by Euplotes aediculatus telomerase at various concentrations of the nucleotide substrates dGTP and dTTP. The maximum processivity (approximately 3 repeats) was observed at approximately 100 microM of each dNTP. Processivity decreased as the dNTP concentrations were reduced and, surprisingly, as the concentration of dGTP was increased....
متن کاملDevelopmentally Programmed Gene Elimination in Euplotes crassus Facilitates a Switch in the Telomerase Catalytic Subunit
The primary function of telomerase is to maintain preexisting telomere tracts. In the ciliate Euplotes crassus, however, telomerase RNP structure and substrate recognition are altered during macronuclear development to facilitate de novo telomere addition. We found that E. crassus harbors three TERT genes encoding the telomerase catalytic subunit that not only vary in their nucleotide and predi...
متن کاملThe telomerase-associated protein p43 is involved in anchoring telomerase in the nucleus.
Telomere replication of eukaryotic chromosomes is achieved by a specialized enzyme, the telomerase. Although the biochemistry of end-replication is well understood, little is known about the organization of the end-replication machinery, its regulation throughout the cell cycle or the biological function of the telomerase-associated proteins. Here we investigate the function of the telomerase-a...
متن کاملPurification of telomerase from Euplotes aediculatus:
Telomerase is a ribonucleoprotein enzyme that uses its internal RNA moiety as a template for synthesis of telomeric repeats at chromosome ends. Here we report the purification of'telomerase from Euplotes aediculatus by affinity chromatography with antisense 2'-O-methyl oligonucleotides, a method that was developed for small nuclear ribonucleoprotein particles (snRNPs). Elution of bound ribonucl...
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ژورنال
عنوان ژورنال: RNA
سال: 2004
ISSN: 1355-8382
DOI: 10.1261/rna.7400704